vitro caco 2 cell permeability  (ATCC)

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Western diet-induced increase in colonic bile acids compromises epithelial barrier in nonalcoholic steatohepatitis

doi: 10.1096/fj.201902687R

Figure Lengend Snippet: Sevelamer protects epithelial barrier from bile acid-induced increase in paracellular permeability. A,B, Paracellularflux of 4 kDa FITC-Dextran across a Caco2 monolayer treated with individual bile acid (BAs) or BAs mixed with sevelamer (Sev). Fully differentiated Caco-2 cell monolayers were treated with individual BAs or BAs premixed with sevelamer for 2 hours in the presence of 4 kDa FITC-Dextran. FITC-dextran flux was determined by measuring the fluorescence intensity of media in the basal chamber. C-D, Transepithelial electrical resistance (TEER) of Caco2 monolayers treated with individual BAs or BAs mixed with sevelamer. TEERwas measured before adding BAs and again after 2-hours incubation. TEER readings are reported as the normalized values after 2-hours incubation. E, Fold change in the intestinal permeability to FITC-dextran in control and CDCA-fed mice (n = 3–4 mice per cohort). CA, cholic acid; CDCA, chenodeoxycholic acid; TCA, taurocholic acid; TDCA, taurodeoxycholic acid; and LCA, lithocholic acid. Data are representative means ± SEM of at least four individual monolayer inserts. *P < .05 HBSS or methanol vs BA-treated wells. #P < .05 BA vs BA plus sevelamer-treated wells

Article Snippet: Cell culture and in vitro permeability assay Caco2 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Permeability, Fluorescence, Incubation, Control